{"id":115,"date":"2025-03-27T03:04:39","date_gmt":"2025-03-27T03:04:39","guid":{"rendered":"http:\/\/drtem.cn\/?p=115"},"modified":"2025-08-08T14:06:04","modified_gmt":"2025-08-08T06:06:04","slug":"deepseek%e7%99%bb%e9%99%86%e8%8b%8f%e5%b7%9e%e5%be%b7%e9%94%90%e7%89%b9%e8%83%bd%e5%b9%b2%e4%bb%80%e4%b9%88%ef%bc%9f%e5%9b%9e%e7%ad%94%e5%86%b7%e5%86%bb%e7%94%b5%e9%95%9c%e6%95%b0%e6%8d%ae%e8%a7%a3","status":"publish","type":"post","link":"https:\/\/www.drtem.cn\/en\/2025\/03\/27\/deepseek%e7%99%bb%e9%99%86%e8%8b%8f%e5%b7%9e%e5%be%b7%e9%94%90%e7%89%b9%e8%83%bd%e5%b9%b2%e4%bb%80%e4%b9%88%ef%bc%9f%e5%9b%9e%e7%ad%94%e5%86%b7%e5%86%bb%e7%94%b5%e9%95%9c%e6%95%b0%e6%8d%ae%e8%a7%a3\/","title":{"rendered":"DeepSeek at DRTem: Can It Handle Technical Details of Cryo-EM Data Analysis?"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"115\" class=\"elementor elementor-115\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2be9f42f e-flex e-con-boxed e-con e-parent\" data-id=\"2be9f42f\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4b03fa39 elementor-widget elementor-widget-text-editor\" data-id=\"4b03fa39\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-size: 14pt; color: #3366ff;\"><strong><b>\u4e00\u3001<\/b><\/strong><strong><b>\u4e3a\u4ec0\u4e48\u5728\u51b7\u51bb\u7535\u955c\u4e09\u7ef4\u91cd\u6784\u7684\u8fc7\u7a0b\u4e2d\u9700\u8981mask<\/b><\/strong><\/span><\/p><p>\u5728\u51b7\u51bb\u7535\u955c\uff08cryo-EM\uff09\u4e09\u7ef4\u91cd\u6784\u8fc7\u7a0b\u4e2d\uff0c**\u63a9\u6a21\uff08mask\uff09**\u7684\u4f7f\u7528\u662f\u4e00\u4e2a\u5173\u952e\u6b65\u9aa4\uff0c\u4e3b\u8981\u76ee\u7684\u662f\u901a\u8fc7\u9650\u5236\u8ba1\u7b97\u533a\u57df\u3001\u6392\u9664\u5e72\u6270\u4fe1\u53f7\u548c\u566a\u58f0\uff0c\u4ece\u800c\u63d0\u5347\u91cd\u6784\u7ed3\u679c\u7684\u7cbe\u5ea6\u548c\u5206\u8fa8\u7387\u3002\u4ee5\u4e0b\u662f\u5176\u6838\u5fc3\u4f5c\u7528\u548c\u6280\u672f\u539f\u7406\u7684\u8be6\u7ec6\u89e3\u91ca\uff1a<\/p><ol><li><strong><b> \u6291\u5236\u566a\u58f0\u4e0e\u80cc\u666f\u5e72\u6270<\/b><\/strong><b><\/b><\/li><\/ol><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><strong><b>\u51b7\u51bb\u7535\u955c\u56fe\u50cf\u7279\u70b9<\/b><\/strong>\uff1a\u5355\u9897\u7c92\u51b7\u51bb\u7535\u955c\u7684\u539f\u59cb\u56fe\u50cf\u4fe1\u566a\u6bd4\uff08SNR\uff09\u6781\u4f4e\uff08\u901a\u5e38 &lt; 0.1\uff09\uff0c\u4e14\u76ee\u6807\u9897\u7c92\uff08\u5982\u86cb\u767d\u8d28\uff09\u53ef\u80fd\u4ec5\u5360\u56fe\u50cf\u7684\u6781\u5c0f\u533a\u57df\uff0c\u5927\u90e8\u5206\u80cc\u666f\u662f\u51b0\u5c42\u6216\u7f13\u51b2\u6db2\u7684\u566a\u58f0\u3002<\/li><li><b><\/b><strong><b>Mask\u7684\u4f5c\u7528<\/b><\/strong>\uff1a<br \/><ul style=\"list-style-type: circle;\"><li><b><\/b><strong><b>\u7269\u7406\u9694\u79bb<\/b><\/strong>\uff1a\u901a\u8fc7\u5b9a\u4e49\u76ee\u6807\u9897\u7c92\u7684\u8fb9\u754c\uff0c\u5c06\u8ba1\u7b97\u96c6\u4e2d\u5728\u9897\u7c92\u672c\u8eab\uff0c\u6392\u9664\u5468\u56f4\u51b0\u5c42\u548c\u80cc\u666f\u566a\u58f0\u7684\u5e72\u6270\u3002<\/li><li><b><\/b><strong><b>\u6291\u5236\u9ad8\u9891\u566a\u58f0<\/b><\/strong>\uff1a\u5728\u5085\u91cc\u53f6\u7a7a\u95f4\uff08\u91cd\u6784\u7684\u6838\u5fc3\u8ba1\u7b97\u57df\uff09\u4e2d\uff0c\u9ad8\u9891\u566a\u58f0\u4f1a\u6c61\u67d3\u4fe1\u53f7\uff0cmask\u80fd\u9650\u5236\u6709\u6548\u4fe1\u53f7\u7684\u8303\u56f4\uff0c\u907f\u514d\u566a\u58f0\u88ab\u8bef\u8ba4\u4e3a\u7ed3\u6784\u4fe1\u606f\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><ol start=\"2\"><li><strong><b> \u63d0\u9ad8\u8ba1\u7b97\u6548\u7387<\/b><\/strong><\/li><\/ol><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u8ba1\u7b97\u8d44\u6e90\u4f18\u5316<\/b><\/strong>\uff1a\u4e09\u7ef4\u91cd\u6784\u6d89\u53ca\u5927\u91cf\u8fed\u4ee3\u8ba1\u7b97\uff08\u5982\u6295\u5f71\u5339\u914d\u3001\u5085\u91cc\u53f6\u53cd\u6f14\uff09\uff0c\u82e5\u5bf9\u5168\u56fe\u8fdb\u884c\u8ba1\u7b97\uff0c\u4f1a\u6d6a\u8d39\u7b97\u529b\u5728\u65e0\u610f\u4e49\u7684\u80cc\u666f\u533a\u57df\u3002<\/li><li><b><\/b><strong><b>\u52a8\u6001\u805a\u7126<\/b><\/strong>\uff1a\u5728\u8fed\u4ee3\u8fc7\u7a0b\u4e2d\uff0cmask\u53ef\u9010\u6b65\u7f29\u5c0f\u5230\u9897\u7c92\u7684\u6838\u5fc3\u533a\u57df\uff08\u4f8b\u5982\u5c40\u90e8\u4f18\u5316\u65f6\uff09\uff0c\u8fdb\u4e00\u6b65\u51cf\u5c11\u8ba1\u7b97\u91cf\u3002<\/li><\/ul><\/li><\/ul><ol start=\"3\"><li><strong><b> \u907f\u514d\u8fb9\u7f18\u4f2a\u5f71\uff08Edge Artifacts\uff09<\/b><\/strong><\/li><\/ol><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u56fe\u50cf\u8fb9\u7f18\u7684\u5e72\u6270<\/b><\/strong>\uff1a\u9897\u7c92\u8fb9\u7f18\u7684\u622a\u65ad\u4f1a\u5bfc\u81f4\u5085\u91cc\u53f6\u53d8\u6362\u65f6\u7684\u201c\u632f\u94c3\u6548\u5e94\u201d\uff08\u7c7b\u4f3c\u6ce2\u7eb9\u7684\u4f2a\u5f71\uff09\uff0c\u5f71\u54cd\u5206\u8fa8\u7387\u3002<\/li><li><b><\/b><strong><b>\u89e3\u51b3\u65b9\u6848<\/b><\/strong>\uff1a<\/li><\/ul><\/li><\/ul><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li><strong><b>\u8f6f\u8fb9\u7f18mask<\/b><\/strong>\uff1a\u7528\u6e10\u53d8\u8fb9\u7f18\u7684mask\uff08\u5982\u4f59\u5f26\u6e10\u53d8\uff09\u5e73\u6ed1\u8fc7\u6e21\uff0c\u51cf\u5c11\u786c\u8fb9\u754c\u5bfc\u81f4\u7684\u4f2a\u5f71\u3002<\/li><li><strong><b>\u6570\u636e\u5b8c\u6574\u6027<\/b><\/strong>\uff1a\u9632\u6b62\u8fb9\u7f18\u566a\u58f0\u88ab\u8bef\u5224\u4e3a\u771f\u5b9e\u7ed3\u6784\uff08\u4f8b\u5982\u51b0\u5c42\u4e2d\u7684\u7ed3\u6676\u4f2a\u5f71\uff09\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><ol start=\"4\"><li><strong><b> \u652f\u6301\u5206\u7c7b\u4e0e\u5bf9\u9f50<\/b><\/strong><br \/><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u9897\u7c92\u7684\u5f02\u8d28\u6027<\/b><\/strong>\uff1a\u6837\u54c1\u4e2d\u53ef\u80fd\u5b58\u5728\u6784\u8c61\u4e0d\u540c\u7684\u9897\u7c92\uff08\u5982\u86cb\u767d\u8d28\u7684\u4e0d\u540c\u72b6\u6001\uff09\uff0cmask\u80fd\u5e2e\u52a9\u7b97\u6cd5\u4e13\u6ce8\u4e8e\u76ee\u6807\u533a\u57df\u7684\u7279\u5f81\uff0c\u63d0\u5347\u5206\u7c7b\uff083D classification\uff09\u7684\u51c6\u786e\u6027\u3002<\/li><li><b><\/b><strong><b>\u5bf9\u9f50\u7cbe\u5ea6<\/b><\/strong>\uff1a\u5728\u6295\u5f71\u5339\u914d\uff08projection matching\uff09\u4e2d\uff0cmask\u80fd\u6392\u9664\u80cc\u666f\u5e72\u6270\uff0c\u4f7f\u65cb\u8f6c\u548c\u5e73\u79fb\u53c2\u6570\u7684\u4f18\u5316\u66f4\u7a33\u5b9a\u3002<\/li><\/ul><\/li><\/ol><ol start=\"5\"><li><strong><b> \u63d0\u5347\u5206\u8fa8\u7387<\/b><\/strong><\/li><\/ol><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u4fe1\u53f7\u805a\u7126<\/b><\/strong>\uff1a\u5728\u540e\u671f\u7684\u9ad8\u5206\u8fa8\u7387\u4f18\u5316\u9636\u6bb5\uff08\u5982Bayesian polishing\u6216\u5c40\u90e8\u7ec6\u5316\uff09\uff0cmask\u4f1a\u4e25\u683c\u9650\u5236\u5230\u9897\u7c92\u7684\u6838\u5fc3\u533a\u57df\uff0c\u907f\u514d\u566a\u58f0\u5728\u9ad8\u9891\u4fe1\u53f7\u4e2d\u5360\u4e3b\u5bfc\u3002<\/li><li><b><\/b><strong><b>\u5206\u8fa8\u7387\u7a81\u7834<\/b><\/strong>\uff1a\u4f8b\u5982\u5728\u539f\u5b50\u7ea7\u5206\u8fa8\u7387\uff08&lt;3\u00c5\uff09\u7684\u91cd\u6784\u4e2d\uff0cmask\u7684\u7cbe\u51c6\u5e94\u7528\u662f\u533a\u5206\u4fa7\u94fe\u7ec6\u8282\u7684\u5173\u952e\u3002<\/li><\/ul><\/li><\/ul><ol start=\"6\"><li><strong><b> \u52a8\u6001\u8c03\u6574\u4e0e\u5c40\u90e8\u4f18\u5316<\/b><\/strong><\/li><\/ol><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u8fed\u4ee3\u8fc7\u7a0b\u4e2d\u7684mask\u6f14\u5316<\/b><\/strong>\uff1a<br \/><ul style=\"list-style-type: circle;\"><li><b><\/b><strong><b>\u521d\u59cbmask<\/b><\/strong>\uff1a\u901a\u5e38\u8f83\u5927\uff0c\u6db5\u76d6\u6574\u4e2a\u9897\u7c92\u548c\u90e8\u5206\u5468\u56f4\u533a\u57df\u3002<\/li><li><b><\/b><strong><b>\u7ec6\u5316mask<\/b><\/strong>\uff1a\u968f\u7740\u91cd\u6784\u7cbe\u5ea6\u7684\u63d0\u5347\uff0cmask\u9010\u6b65\u7f29\u5c0f\u5230\u66f4\u7cbe\u786e\u7684\u9897\u7c92\u8fb9\u754c\uff0c\u751a\u81f3\u9488\u5bf9\u4e9a\u7ed3\u6784\uff08\u5982\u67d4\u6027\u533a\u57df\uff09\u4f7f\u7528\u5c40\u90e8mask\u3002<\/li><\/ul><\/li><li><b><\/b><strong><b>\u67d4\u6027\u62df\u5408\uff08Flexible fitting\uff09<\/b><\/strong>\uff1a\u5bf9\u4e8e\u52a8\u6001\u7ed3\u6784\uff0cmask\u53ef\u5206\u5272\u4e3a\u591a\u4e2a\u533a\u57df\uff0c\u5206\u522b\u4f18\u5316\u4e0d\u540c\u90e8\u5206\u7684\u8fd0\u52a8\u548c\u53d8\u5f62\u3002<\/li><\/ul><\/li><\/ul><p>\u00a0 \u00a0 \u00a07.<strong><b>\u6280\u672f\u5b9e\u73b0\u793a\u4f8b<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><strong><b>\u5982\u4f55\u751f\u6210mask?<\/b><\/strong><\/li><\/ul><\/li><\/ul><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li><b><\/b><strong><b>\u521d\u59cbmask<\/b><\/strong>\uff1a\u6839\u636e\u9897\u7c92\u7684\u7c97\u7565\u6a21\u578b\uff08\u4f4e\u5206\u8fa8\u7387\u5bc6\u5ea6\u56fe\uff09\u81ea\u52a8\u751f\u6210\u7403\u5f62\u6216\u692d\u7403\u5f62mask\u3002<\/li><li><b><\/b><strong><b>\u9608\u503c\u6cd5<\/b><\/strong>\uff1a\u57fa\u4e8e\u5bc6\u5ea6\u56fe\u7684\u9608\u503c\u5206\u5272\uff08\u5982UCSF Chimera\u4e2dvop\u547d\u4ee4\uff09\u3002<\/li><li><b><\/b><strong><b>\u673a\u5668\u5b66\u4e60\u8f85\u52a9<\/b><\/strong>\uff1a\u4f7f\u7528\u6df1\u5ea6\u5b66\u4e60\u6a21\u578b\uff08\u5982Topaz\u6216CRYOLO\uff09\u81ea\u52a8\u8bc6\u522b\u9897\u7c92\u8fb9\u754c\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u8f6fmask vs \u786cmask<\/b><\/strong>\uff1a<\/li><\/ul><\/li><\/ul><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li>\u786cmask\uff08\u4e8c\u503c\u5316\uff0c0\u62161\uff09\u53ef\u80fd\u5bfc\u81f4\u8fb9\u754c\u4f2a\u5f71\u3002<\/li><li>\u8f6fmask\uff08\u6e10\u53d8\u6743\u91cd\uff09\u66f4\u7b26\u5408\u771f\u5b9e\u4fe1\u53f7\u5206\u5e03\uff0c\u51cf\u5c11\u9ad8\u9891\u4f2a\u5f71\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><p><strong><b>\u603b\u7ed3<\/b><\/strong><\/p><p><strong><b>Mask\u7684\u672c\u8d28\u662f\u201c\u4fe1\u566a\u6bd4\u653e\u5927\u5668\u201d<\/b><\/strong>\uff1a<br \/>\u901a\u8fc7\u6392\u9664\u80cc\u666f\u566a\u58f0\u3001\u805a\u7126\u76ee\u6807\u4fe1\u53f7\uff0cmask\u5728\u51b7\u51bb\u7535\u955c\u91cd\u6784\u4e2d\u626e\u6f14\u4e86\u201c\u4fe1\u53f7\u8fc7\u6ee4\u5668\u201d\u548c\u201c\u8ba1\u7b97\u52a0\u901f\u5668\u201d\u7684\u53cc\u91cd\u89d2\u8272\u3002\u5c24\u5176\u5728\u8ffd\u6c42\u8fd1\u539f\u5b50\u5206\u8fa8\u7387\u65f6\uff0cmask\u7684\u7cbe\u786e\u8bbe\u8ba1\u548c\u52a8\u6001\u8c03\u6574\u662f\u7a81\u7834\u6280\u672f\u74f6\u9888\u7684\u5173\u952e\u6b65\u9aa4\u3002<\/p><p><span style=\"font-size: 14pt; color: #3366ff;\"><strong><b>\u4e8c\u3001<\/b><\/strong><strong><b>RELION\u7684Class3D\u4efb\u52a1\u4e2d\u7684local mask\u662f\u6307\u4ec0\u4e48<\/b><\/strong><\/span><\/p><p>\u5728RELION\u7684<strong><b>Class3D\u4efb\u52a1<\/b><\/strong>\u4e2d\uff0c**local mask\uff08\u5c40\u90e8\u63a9\u6a21\uff09**\u662f\u4e00\u4e2a\u5173\u952e\u6982\u5ff5\uff0c\u4e3b\u8981\u7528\u4e8e\u5904\u7406\u7ed3\u6784\u5f02\u8d28\u6027\uff08structural heterogeneity\uff09\u95ee\u9898\u3002\u5176\u6838\u5fc3\u76ee\u7684\u662f\u5728\u4e09\u7ef4\u5206\u7c7b\uff083D classification\uff09\u8fc7\u7a0b\u4e2d\uff0c<strong><b>\u9650\u5236\u7b97\u6cd5\u5bf9\u7279\u5b9a\u5c40\u90e8\u533a\u57df\u7684\u5173\u6ce8<\/b><\/strong>\uff0c\u4ece\u800c\u66f4\u7cbe\u51c6\u5730\u6355\u6349\u76ee\u6807\u7ed3\u6784\u7684\u7ec6\u5fae\u6784\u8c61\u53d8\u5316\u6216\u52a8\u6001\u7279\u5f81\u3002<\/p><ol><li><strong><b> Local Mask\u7684\u5b9a\u4e49<\/b><\/strong><br \/><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u5c40\u90e8\u63a9\u6a21<\/b><\/strong>\u662f\u4e00\u4e2a\u4e8c\u8fdb\u5236\u6216\u8f6f\u8fb9\u754c\u7684\u533a\u57df\u5b9a\u4e49\uff0c\u4ec5\u8986\u76d6\u76ee\u6807\u7ed3\u6784\u7684<strong><b>\u7279\u5b9a\u90e8\u5206<\/b><\/strong>\uff08\u4f8b\u5982\u86cb\u767d\u8d28\u7684\u67d0\u4e2a\u7ed3\u6784\u57df\u3001\u67d4\u6027\u533a\u57df\u6216\u914d\u4f53\u7ed3\u5408\u4f4d\u70b9\uff09\uff0c\u800c\u975e\u6574\u4e2a\u9897\u7c92\u3002<\/li><li>\u5b83\u901a\u8fc7\u7ea6\u675f\u7b97\u6cd5\u5728\u8fed\u4ee3\u4f18\u5316\u65f6<strong><b>\u53ea\u5206\u6790\u63a9\u6a21\u5185\u7684\u4fe1\u53f7<\/b><\/strong>\uff0c\u5ffd\u7565\u63a9\u6a21\u5916\u7684\u533a\u57df\uff08\u5982\u6eb6\u5242\u533a\u3001\u51b0\u5c42\u6216\u5176\u4ed6\u65e0\u5173\u7ed3\u6784\uff09\u3002<\/li><\/ul><\/li><\/ol><ol start=\"2\"><li><strong><b> \u4e3a\u4ec0\u4e48\u9700\u8981Local Mask\uff1f<\/b><\/strong><\/li><\/ol><p style=\"padding-left: 40px;\"><strong><b>(1) \u89e3\u51b3\u5c40\u90e8\u5f02\u8d28\u6027\uff08Local Heterogeneity\uff09<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u95ee\u9898\u80cc\u666f<\/b><\/strong>\uff1a\u8bb8\u591a\u751f\u7269\u5927\u5206\u5b50\uff08\u5982\u75c5\u6bd2\u8863\u58f3\u3001\u6838\u7cd6\u4f53\u7b49\uff09\u53ef\u80fd\u4ec5\u90e8\u5206\u533a\u57df\u5b58\u5728\u6784\u8c61\u53d8\u5316\uff08\u5982\u7ed3\u6784\u57df\u8fd0\u52a8\u3001\u4fa7\u94fe\u7ffb\u8f6c\uff09\uff0c\u800c\u5176\u4ed6\u533a\u57df\u4fdd\u6301\u521a\u6027\u3002<\/li><li><b><\/b><strong><b>\u5168\u5c40\u63a9\u6a21\u7684\u5c40\u9650\u6027<\/b><\/strong>\uff1a\u82e5\u4f7f\u7528\u5168\u5c40\u63a9\u6a21\uff08\u8986\u76d6\u6574\u4e2a\u9897\u7c92\uff09\uff0c\u7b97\u6cd5\u53ef\u80fd\u56e0\u8fc7\u5ea6\u5173\u6ce8\u7a33\u5b9a\u533a\u57df\u800c\u5ffd\u7565\u5c40\u90e8\u53d8\u5316\uff0c\u5bfc\u81f4\u5206\u7c7b\u6a21\u7cca\u6216\u5206\u8fa8\u7387\u635f\u5931\u3002<\/li><li><b><\/b><strong><b>Local Mask\u7684\u4f18\u52bf<\/b><\/strong>\uff1a<br \/><ul style=\"list-style-type: circle;\"><li><b><\/b><strong><b>\u805a\u7126\u52a8\u6001\u533a\u57df<\/b><\/strong>\uff1a\u5f3a\u5236\u7b97\u6cd5\u4ec5\u5206\u6790\u76ee\u6807\u533a\u57df\u7684\u53d8\u5316\uff0c\u63d0\u9ad8\u5206\u7c7b\u7075\u654f\u5ea6\u3002<\/li><li><b><\/b><strong><b>\u51cf\u5c11\u566a\u58f0\u5e72\u6270<\/b><\/strong>\uff1a\u6392\u9664\u7a33\u5b9a\u533a\u57df\u548c\u80cc\u666f\u7684\u5e72\u6270\uff0c\u63d0\u5347\u4fe1\u566a\u6bd4\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u907f\u514d\u8fc7\u62df\u5408\uff08Overfitting\uff09<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li>\u5f53\u5168\u5c40\u63a9\u6a21\u5305\u542b\u8fc7\u591a\u566a\u58f0\u533a\u57df\u65f6\uff0c\u7b97\u6cd5\u53ef\u80fd\u5c06\u566a\u58f0\u8bef\u8ba4\u4e3a\u7ed3\u6784\u4fe1\u53f7\uff0c\u5bfc\u81f4\u5206\u7c7b\u9519\u8bef\u3002Local Mask\u901a\u8fc7\u9650\u5236\u533a\u57df\uff0c\u964d\u4f4e\u8fc7\u62df\u5408\u98ce\u9669\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) \u8ba1\u7b97\u6548\u7387\u4f18\u5316<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li>\u5c40\u90e8\u63a9\u6a21\u663e\u8457\u51cf\u5c11\u8ba1\u7b97\u91cf\uff08\u4ec5\u5904\u7406\u5c40\u90e8\u533a\u57df\uff09\uff0c\u5c24\u5176\u9002\u7528\u4e8e\u5927\u89c4\u6a21\u6570\u636e\u96c6\u6216\u9ad8\u5206\u8fa8\u7387\u4f18\u5316\u3002<\/li><\/ul><\/li><\/ul><ol start=\"3\"><li><strong><b> Local Mask\u5728Class3D\u4e2d\u7684\u5177\u4f53\u5e94\u7528<\/b><\/strong><\/li><\/ol><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u5206\u7c7b\u6d41\u7a0b<\/b><\/strong>\uff1a<br \/><ul style=\"list-style-type: circle;\"><li><b><\/b><strong><b>\u521d\u59cb\u6a21\u578b\u751f\u6210<\/b><\/strong>\uff1a\u4f7f\u7528\u5168\u5c40\u63a9\u6a21\u8fdb\u884c\u521d\u6b65\u4f4e\u5206\u8fa8\u7387\u5206\u7c7b\u3002<\/li><li><b><\/b><strong><b>\u8bc6\u522b\u52a8\u6001\u533a\u57df<\/b><\/strong>\uff1a\u901a\u8fc7\u5206\u6790\u521d\u6b65\u5206\u7c7b\u7ed3\u679c\uff0c\u786e\u5b9a\u9700\u8981\u5c40\u90e8\u4f18\u5316\u7684\u533a\u57df\uff08\u5982\u67d4\u6027\u73af\u3001\u914d\u4f53\u7ed3\u5408\u4f4d\u70b9\uff09\u3002<\/li><li><b><\/b><strong><b>\u751f\u6210Local Mask<\/b><\/strong>\uff1a\u9488\u5bf9\u52a8\u6001\u533a\u57df\u5b9a\u4e49\u63a9\u6a21\uff08\u53ef\u901a\u8fc7\u624b\u52a8\u7ed8\u5236\u6216\u81ea\u52a8\u9608\u503c\u5206\u5272\uff09\u3002<\/li><li><b><\/b><strong><b>\u8fed\u4ee3\u4f18\u5316<\/b><\/strong>\uff1a\u5728Class3D\u4efb\u52a1\u4e2d\u52a0\u8f7dLocal Mask\uff0c\u7b97\u6cd5\u4ec5\u4f18\u5316\u63a9\u6a21\u5185\u7684\u5bc6\u5ea6\uff0c\u540c\u65f6\u56fa\u5b9a\u5176\u4ed6\u533a\u57df\u7684\u7ed3\u6784\u3002<\/li><\/ul><\/li><li><b><\/b><strong><b>\u53c2\u6570\u8bbe\u7f6e\u793a\u4f8b\uff08RELION\u8bed\u6cd5\uff09<\/b><\/strong>\uff1a<\/li><\/ul><p style=\"padding-left: 40px;\"># \u5728Class3D\u7684STAR\u6587\u4ef6\u4e2d\u6307\u5b9aLocal Mask<\/p><p style=\"padding-left: 40px;\">local_mask_file = my_local_mask.mrc<\/p><ol><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li><strong><b>\u8f6f\u8fb9\u754c\u5904\u7406<\/b><\/strong>\uff1a\u901a\u5e38\u5efa\u8bae\u4f7f\u7528\u6e10\u53d8\u8fb9\u7f18\uff08soft mask\uff09\u4ee5\u51cf\u5c11\u4f2a\u5f71\u3002<\/li><\/ul><\/li><\/ol><p style=\"padding-left: 40px;\">4. <strong><b>\u5982\u4f55\u751f\u6210Local Mask\uff1f<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><strong><b>\u624b\u52a8\u751f\u6210<\/b><\/strong>\uff1a<br \/>\u4f7f\u7528\u53ef\u89c6\u5316\u5de5\u5177\uff08\u5982UCSF Chimera\u3001ChimeraX\u6216EMAN2\uff09\u624b\u52a8\u7ed8\u5236\u76ee\u6807\u533a\u57df\u3002<br \/><strong><b>\u6b65\u9aa4<\/b><\/strong>\uff1a<br \/><ul style=\"list-style-type: circle;\"><li>\u52a0\u8f7d\u521d\u6b65\u91cd\u6784\u7684\u4f4e\u5206\u8fa8\u7387\u5bc6\u5ea6\u56fe\u3002<\/li><li>\u7528\u9608\u503c\u5206\u5272\u786e\u5b9a\u52a8\u6001\u533a\u57df\u3002<\/li><li>\u4f7f\u7528\u753b\u7b14\u5de5\u5177\u6807\u8bb0\u533a\u57df\u5e76\u5bfc\u51fa\u4e3aMRC\u6587\u4ef6\u3002<\/li><\/ul><\/li><li><strong><b>\u81ea\u52a8\u751f\u6210<\/b><\/strong>\uff1a<br \/><ul style=\"list-style-type: circle;\"><li><strong><b>\u9608\u503c\u5206\u5272<\/b><\/strong>\uff1a\u901a\u8fc7\u5bc6\u5ea6\u503c\u9608\u503c\u63d0\u53d6\u7279\u5b9a\u533a\u57df\uff08\u5982relion_mask_create\u5de5\u5177\uff09\u3002<\/li><li><strong><b>\u673a\u5668\u5b66\u4e60\u8f85\u52a9<\/b><\/strong>\uff1a\u5229\u7528\u6df1\u5ea6\u5b66\u4e60\u6a21\u578b\uff08\u5982Topaz\u6216cryoDRGN\uff09\u9884\u6d4b\u52a8\u6001\u533a\u57df\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><\/li><\/ul><p>\u00a0 \u00a0 \u00a0 \u00a05. <strong><b>Local Mask vs. Global Mask<\/b><\/strong><\/p><table style=\"width: 45.3448%; height: 156px;\"><tbody><tr><td style=\"width: 25.4509%;\"><strong><b>\u7279\u5f81<\/b><\/strong><\/td><td style=\"width: 34.1416%;\"><strong><b>Local Mask<\/b><\/strong><\/td><td style=\"width: 86.5034%;\"><strong><b>Global Mask<\/b><\/strong><\/td><\/tr><tr><td style=\"width: 25.4509%;\"><strong><b>\u8986\u76d6\u8303\u56f4<\/b><\/strong><\/td><td style=\"width: 34.1416%;\">\u4ec5\u76ee\u6807\u7ed3\u6784\u7684\u5c40\u90e8\u533a\u57df<\/td><td style=\"width: 86.5034%;\">\u6574\u4e2a\u9897\u7c92\uff08\u5305\u62ec\u6eb6\u5242\u533a\uff09<\/td><\/tr><tr><td style=\"width: 25.4509%;\"><strong><b>\u9002\u7528\u573a\u666f<\/b><\/strong><\/td><td style=\"width: 34.1416%;\">\u5c40\u90e8\u5f02\u8d28\u6027\uff08\u5982\u7ed3\u6784\u57df\u8fd0\u52a8\uff09<\/td><td style=\"width: 86.5034%;\">\u6574\u4f53\u6784\u8c61\u53d8\u5316\uff08\u5982\u5168\u9897\u7c92\u65cb\u8f6c\uff09<\/td><\/tr><tr><td style=\"width: 25.4509%;\"><strong><b>\u8ba1\u7b97\u91cf<\/b><\/strong><\/td><td style=\"width: 34.1416%;\">\u8f83\u5c0f\uff08\u4ec5\u5904\u7406\u5c40\u90e8\u4fe1\u53f7\uff09<\/td><td style=\"width: 86.5034%;\">\u8f83\u5927\uff08\u5904\u7406\u5168\u56fe\uff09<\/td><\/tr><tr><td style=\"width: 25.4509%;\"><strong><b>\u5206\u8fa8\u7387\u4f18\u5316<\/b><\/strong><\/td><td style=\"width: 34.1416%;\">\u66f4\u6613\u6355\u6349\u9ad8\u9891\u7ec6\u8282\uff08\u5982\u4fa7\u94fe\u6784\u8c61\uff09<\/td><td style=\"width: 86.5034%;\">\u53ef\u80fd\u53d7\u80cc\u666f\u566a\u58f0\u5f71\u54cd<\/td><\/tr><\/tbody><\/table><p>\u00a0 \u00a0 \u00a0 \u00a0 6. <strong><b>\u5b9e\u9645\u6848\u4f8b<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul><li><b><\/b><strong><b>\u75c5\u6bd2\u8863\u58f3\u7684\u6784\u8c61\u5206\u6790<\/b><\/strong>\uff1a<ul><li>\u82e5\u75c5\u6bd2\u8863\u58f3\u7684\u9876\u70b9\u533a\u57df\u5b58\u5728\u5f00\u5408\u8fd0\u52a8\uff0c\u53ef\u9488\u5bf9\u9876\u70b9\u5b9a\u4e49Local Mask\uff0c\u5206\u7c7b\u65f6\u4ec5\u4f18\u5316\u8be5\u533a\u57df\uff0c\u4ece\u800c\u533a\u5206\u5f00\u653e\/\u95ed\u5408\u72b6\u6001\u3002<\/li><\/ul><\/li><li><b><\/b><strong><b>\u819c\u86cb\u767d\u7684\u8de8\u819c\u533a\u67d4\u6027<\/b><\/strong>\uff1a<ul><li>\u819c\u86cb\u767d\u7684\u80de\u5916\u57df\u53ef\u80fd\u7a33\u5b9a\uff0c\u800c\u8de8\u819c\u533a\u5b58\u5728\u6784\u8c61\u6ce2\u52a8\u3002Local Mask\u53ef\u805a\u7126\u8de8\u819c\u533a\uff0c\u907f\u514d\u80de\u5916\u57df\u7684\u7a33\u5b9a\u4fe1\u53f7\u63a9\u76d6\u52a8\u6001\u53d8\u5316\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><p>\u00a0 \u00a0 \u00a0 \u00a07.<strong><b> \u6ce8\u610f\u4e8b\u9879<\/b><\/strong><\/p><ol><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u63a9\u6a21\u8fb9\u754c\u8fc7\u6e21<\/b><\/strong>\uff1a\u786c\u8fb9\u754c\uff08\u4e8c\u503c\u5316\uff09\u53ef\u80fd\u5bfc\u81f4\u4f2a\u5f71\uff0c\u5efa\u8bae\u4f7f\u7528\u6e10\u53d8\u8fb9\u7f18\uff08soft mask\uff09\u3002<\/li><li><b><\/b><strong><b>\u52a8\u6001\u8c03\u6574<\/b><\/strong>\uff1a\u5728\u8fed\u4ee3\u8fc7\u7a0b\u4e2d\uff0c\u53ef\u6839\u636e\u5206\u7c7b\u7ed3\u679c\u9010\u6b65\u7f29\u5c0f\u6216\u8c03\u6574Local Mask\u8303\u56f4\u3002<\/li><li><b><\/b><strong><b>\u5206\u8fa8\u7387\u5339\u914d<\/b><\/strong>\uff1a\u9ad8\u5206\u8fa8\u7387\u4f18\u5316\u9700\u4f7f\u7528\u66f4\u7cbe\u786e\u7684Local Mask\uff08\u907f\u514d\u5305\u542b\u4f4e\u4fe1\u566a\u6bd4\u533a\u57df\uff09\u3002<\/li><\/ul><\/li><\/ol><p><strong><b>\u603b\u7ed3<\/b><\/strong><\/p><p>\u5728RELION\u7684Class3D\u4efb\u52a1\u4e2d\uff0c<strong><b>Local Mask<\/b><\/strong>\u662f\u4e00\u79cd\u9488\u5bf9\u5c40\u90e8\u7ed3\u6784\u5f02\u8d28\u6027\u7684\u9ad8\u6548\u5de5\u5177\uff0c\u901a\u8fc7\u7ea6\u675f\u7b97\u6cd5\u5173\u6ce8\u7279\u5b9a\u52a8\u6001\u533a\u57df\uff0c\u663e\u8457\u63d0\u5347\u5206\u7c7b\u7cbe\u5ea6\u548c\u5206\u8fa8\u7387\u3002\u5176\u6838\u5fc3\u4ef7\u503c\u5728\u4e8e\uff1a<\/p><ul><li><b><\/b><strong><b>\u7cbe\u51c6\u6355\u83b7\u6784\u8c61\u53d8\u5316<\/b><\/strong>\uff08\u5982\u9176\u6d3b\u6027\u4f4d\u70b9\u7684\u5f00\u95ed\u3001\u914d\u4f53\u7ed3\u5408\u5f15\u8d77\u7684\u5c40\u90e8\u5f62\u53d8\uff09\u3002<\/li><li><b><\/b><strong><b>\u964d\u4f4e\u8ba1\u7b97\u6210\u672c\u4e0e\u8fc7\u62df\u5408\u98ce\u9669<\/b><\/strong>\uff0c\u662f\u5904\u7406\u590d\u6742\u751f\u7269\u5927\u5206\u5b50\u52a8\u6001\u7ed3\u6784\u7684\u5fc5\u5907\u6280\u672f\u3002<\/li><\/ul><p><span style=\"font-size: 14pt; color: #3366ff;\"><strong><b>\u4e09\u3001<\/b><\/strong><strong><b>CryoSPARC\u7684non-uniform refinement\u4efb\u52a1\u7684\u9002\u7528\u573a\u666f\u662f\u4ec0\u4e48<\/b><\/strong><\/span><\/p><p>CryoSPARC\u4e2d\u7684<strong><b>Non-uniform Refinement\uff08\u975e\u5747\u5300\u7ec6\u5316\uff09\u4efb\u52a1\u662f\u4e00\u79cd\u9488\u5bf9\u51b7\u51bb\u7535\u955c\u6570\u636e\u4e2d\u5c40\u90e8\u5f02\u8d28\u6027<\/b><\/strong>\u548c<strong><b>\u52a8\u6001\u6027<\/b><\/strong>\u7684\u9ad8\u7ea7\u4f18\u5316\u65b9\u6cd5\uff0c\u65e8\u5728\u901a\u8fc7\u5141\u8bb8\u4e0d\u540c\u533a\u57df\u72ec\u7acb\u4f18\u5316\u8fd0\u52a8\u53c2\u6570\u548c\u5206\u8fa8\u7387\uff0c\u63d0\u5347\u6574\u4f53\u91cd\u6784\u8d28\u91cf\u3002\u4ee5\u4e0b\u662f\u5176\u9002\u7528\u573a\u666f\u3001\u6280\u672f\u539f\u7406\u53ca\u4f18\u52bf\u7684\u8be6\u7ec6\u5206\u6790\uff1a<\/p><ol><li><strong><b> \u9002\u7528\u573a\u666f<\/b><\/strong><\/li><\/ol><p style=\"padding-left: 40px;\"><strong><b>(1) \u5904\u7406\u5c40\u90e8\u52a8\u6001\u6027\uff08Local Flexibility\uff09<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u95ee\u9898\u80cc\u666f<\/b><\/strong>\uff1a\u751f\u7269\u5927\u5206\u5b50\uff08\u5982\u819c\u86cb\u767d\u3001\u75c5\u6bd2\u8863\u58f3\uff09\u7684\u67d0\u4e9b\u533a\u57df\u53ef\u80fd\u5177\u6709<strong><b>\u67d4\u6027\u6216\u52a8\u6001\u8fd0\u52a8<\/b><\/strong>\uff08\u4f8b\u5982\u7ed3\u6784\u57df\u6446\u52a8\u3001\u4fa7\u94fe\u8fd0\u52a8\uff09\uff0c\u800c\u5176\u4ed6\u533a\u57df\u4fdd\u6301\u521a\u6027\u3002<\/li><li><b><\/b><strong><b>\u4f20\u7edf\u65b9\u6cd5\u7684\u5c40\u9650<\/b><\/strong>\uff1a\u5747\u5300\u7ec6\u5316\uff08Uniform Refinement\uff09\u5047\u8bbe\u6574\u4e2a\u7ed3\u6784\u7684\u8fd0\u52a8\u53c2\u6570\uff08\u5982B\u56e0\u5b50\uff09\u4e00\u81f4\uff0c\u65e0\u6cd5\u51c6\u786e\u6355\u6349\u5c40\u90e8\u52a8\u6001\u6027\u3002<\/li><li><b><\/b><strong><b>Non-uniform Refinement\u7684\u4f5c\u7528<\/b><\/strong>\uff1a<\/li><\/ul><\/li><\/ul><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li>\u5bf9\u6bcf\u4e2a\u5c40\u90e8\u533a\u57df\uff08\u59823D\u4f53\u7d20\u6216\u5b50\u4f53\u79ef\uff09\u72ec\u7acb\u4f18\u5316\u8fd0\u52a8\u53c2\u6570\uff08\u5982\u5404\u5411\u5f02\u6027B\u56e0\u5b50\uff09\uff0c\u6821\u6b63\u5c40\u90e8\u8fd0\u52a8\u6a21\u7cca\u3002<\/li><li>\u63d0\u5347\u52a8\u6001\u533a\u57df\u7684\u5206\u8fa8\u7387\uff0c\u540c\u65f6\u4fdd\u6301\u521a\u6027\u533a\u57df\u7684\u7a33\u5b9a\u6027\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u6821\u6b63\u51b0\u5c42\u6216\u6837\u54c1\u5236\u5907\u5f15\u8d77\u7684\u975e\u5747\u5300\u6027<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u51b0\u5c42\u539a\u5ea6\u4e0d\u5747<\/b><\/strong>\uff1a\u6837\u54c1\u5236\u5907\u4e2d\u51b0\u5c42\u539a\u5ea6\u6216\u65b9\u5411\u6027\u5dee\u5f02\u4f1a\u5bfc\u81f4\u5c40\u90e8\u4fe1\u53f7\u8870\u51cf\u4e0d\u540c\u3002<\/li><li><b><\/b><strong><b>\u89e3\u51b3\u65b9\u6848<\/b><\/strong>\uff1a\u975e\u5747\u5300\u7ec6\u5316\u53ef\u8865\u507f\u5c40\u90e8\u51b0\u5c42\u6548\u5e94\uff0c\u6062\u590d\u771f\u5b9e\u7ed3\u6784\u4fe1\u53f7\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) \u7a81\u7834\u5206\u8fa8\u7387\u74f6\u9888<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u5747\u5300\u7ec6\u5316\u540e\u7684\u505c\u6ede<\/b><\/strong>\uff1a\u5f53\u5747\u5300\u7ec6\u5316\u65e0\u6cd5\u8fdb\u4e00\u6b65\u63d0\u5347\u5206\u8fa8\u7387\u65f6\uff08\u5982\u5361\u57283-4\u00c5\uff09\uff0c\u53ef\u80fd\u56e0\u5c40\u90e8\u52a8\u6001\u6027\u6216\u566a\u58f0\u4e3b\u5bfc\u533a\u57df\u9650\u5236\u4e86\u5168\u5c40\u4f18\u5316\u3002<\/li><li><b><\/b><strong><b>\u975e\u5747\u5300\u7ec6\u5316\u7684\u4f18\u52bf<\/b><\/strong>\uff1a\u901a\u8fc7\u5c40\u90e8\u53c2\u6570\u4f18\u5316\uff0c\u6392\u9664\u4f4e\u4fe1\u566a\u6bd4\u533a\u57df\u7684\u5e72\u6270\uff0c\u805a\u7126\u9ad8\u4fe1\u566a\u6bd4\u533a\u57df\uff0c\u53ef\u80fd\u5c06\u5206\u8fa8\u7387\u63d0\u5347\u81f3\u8fd1\u539f\u5b50\u7ea7\u522b\uff08&lt;3\u00c5\uff09\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(4) \u5904\u7406\u5927\u5206\u5b50\u590d\u5408\u4f53\u7684\u4e0d\u5bf9\u79f0\u6027<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u590d\u5408\u4f53\u7ed3\u6784\u5f02\u8d28\u6027<\/b><\/strong>\uff1a\u4f8b\u5982\u6838\u7cd6\u4f53\u4e0e\u4e0d\u540c\u7ffb\u8bd1\u56e0\u5b50\u7ed3\u5408\u65f6\uff0c\u5c40\u90e8\u6784\u8c61\u5dee\u5f02\u663e\u8457\u3002<\/li><li><b><\/b><strong><b>\u975e\u5747\u5300\u7ec6\u5316<\/b><\/strong>\uff1a\u53ef\u9488\u5bf9\u4e0d\u540c\u4e9a\u57fa\u6216\u7ed3\u5408\u4f4d\u70b9\u72ec\u7acb\u4f18\u5316\uff0c\u907f\u514d\u5168\u5c40\u5e73\u5747\u5316\u5bfc\u81f4\u7684\u7ec6\u8282\u4e22\u5931\u3002<\/li><\/ul><\/li><\/ul><ol start=\"2\"><li><strong><b> \u6280\u672f\u539f\u7406<\/b><\/strong><\/li><\/ol><p style=\"padding-left: 40px;\"><strong><b>(1) \u5c40\u90e8\u8fd0\u52a8\u53c2\u6570\u5efa\u6a21<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u5404\u5411\u5f02\u6027B\u56e0\u5b50<\/b><\/strong>\uff1a\u4e3a\u6bcf\u4e2a3D\u4f53\u7d20\uff08voxel\uff09\u5206\u914d\u72ec\u7acb\u7684B\u56e0\u5b50\uff0c\u6821\u6b63\u5c40\u90e8\u8fd0\u52a8\u6a21\u7cca\u3002<\/li><li><b><\/b><strong><b>\u9891\u7387\u4f9d\u8d56\u7684\u6743\u91cd\u8c03\u6574<\/b><\/strong>\uff1a\u5728\u9ad8\u9891\u4fe1\u53f7\uff08\u9ad8\u5206\u8fa8\u7387\u7ec6\u8282\uff09\u533a\u57df\u964d\u4f4e\u566a\u58f0\u6743\u91cd\uff0c\u589e\u5f3a\u6709\u6548\u4fe1\u53f7\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u5206\u533a\u4f18\u5316\u7b56\u7565<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u5b50\u4f53\u79ef\u5212\u5206<\/b><\/strong>\uff1a\u5c063D\u5bc6\u5ea6\u56fe\u5212\u5206\u4e3a\u591a\u4e2a\u5b50\u533a\u57df\uff0c\u5206\u522b\u4f18\u5316\u5176\u8fd0\u52a8\u53c2\u6570\u548c\u5206\u8fa8\u7387\u3002<\/li><li><b><\/b><strong><b>\u81ea\u9002\u5e94\u5e73\u6ed1\u7ea6\u675f<\/b><\/strong>\uff1a\u9632\u6b62\u8fc7\u5ea6\u62df\u5408\uff0c\u901a\u8fc7\u7a7a\u95f4\u76f8\u5173\u6027\u7ea6\u675f\u76f8\u90bb\u533a\u57df\u7684\u53c2\u6570\u53d8\u5316\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) \u4e0e\u5168\u5c40\u6a21\u578b\u7684\u7ed3\u5408<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li>\u5728\u4fdd\u6301\u5168\u5c40\u7ed3\u6784\u4e00\u81f4\u6027\u7684\u524d\u63d0\u4e0b\uff0c\u5141\u8bb8\u5c40\u90e8\u533a\u57df\u7684\u72ec\u7acb\u4f18\u5316\uff0c\u5e73\u8861\u6574\u4f53\u4e0e\u5c40\u90e8\u7cbe\u5ea6\u3002<\/li><\/ul><\/li><\/ul><ol start=\"3\"><li><strong><b> \u4e0eUniform Refinement\u7684\u5bf9\u6bd4<\/b><\/strong><\/li><\/ol><table style=\"width: 49.0373%;\"><tbody><tr><td style=\"width: 29.0818%;\"><strong><b>\u7279\u5f81<\/b><\/strong><\/td><td style=\"width: 38.8989%;\"><strong><b>Non-uniform Refinement<\/b><\/strong><\/td><td style=\"width: 87.9852%;\"><strong><b>Uniform Refinement<\/b><\/strong><\/td><\/tr><tr><td style=\"width: 29.0818%;\"><strong><b>\u8fd0\u52a8\u6a21\u578b<\/b><\/strong><\/td><td style=\"width: 38.8989%;\">\u5c40\u90e8\u72ec\u7acb\u4f18\u5316\uff08\u5404\u5411\u5f02\u6027B\u56e0\u5b50\uff09<\/td><td style=\"width: 87.9852%;\">\u5168\u5c40\u7edf\u4e00\u53c2\u6570\uff08\u5404\u5411\u540c\u6027B\u56e0\u5b50\uff09<\/td><\/tr><tr><td style=\"width: 29.0818%;\"><strong><b>\u9002\u7528\u573a\u666f<\/b><\/strong><\/td><td style=\"width: 38.8989%;\">\u5c40\u90e8\u52a8\u6001\u6027\u3001\u51b0\u5c42\u4e0d\u5747\u3001\u5206\u8fa8\u7387\u74f6\u9888<\/td><td style=\"width: 87.9852%;\">\u521a\u6027\u7ed3\u6784\u3001\u5747\u4e00\u6837\u54c1<\/td><\/tr><tr><td style=\"width: 29.0818%;\"><strong><b>\u8ba1\u7b97\u91cf<\/b><\/strong><\/td><td style=\"width: 38.8989%;\">\u8f83\u9ad8\uff08\u9700\u5206\u533a\u4f18\u5316\uff09<\/td><td style=\"width: 87.9852%;\">\u8f83\u4f4e<\/td><\/tr><tr><td style=\"width: 29.0818%;\"><strong><b>\u5206\u8fa8\u7387\u6f5c\u529b<\/b><\/strong><\/td><td style=\"width: 38.8989%;\">\u66f4\u9ad8\uff08\u8fd1\u539f\u5b50\u7ea7\uff09<\/td><td style=\"width: 87.9852%;\">\u53d7\u9650\u4e8e\u5168\u5c40\u566a\u58f0<\/td><\/tr><\/tbody><\/table><ol start=\"4\"><li><strong><b> \u4f7f\u7528\u5efa\u8bae<\/b><\/strong><\/li><\/ol><p style=\"padding-left: 40px;\"><strong><b>(1) \u9002\u7528\u6761\u4ef6<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u8f93\u5165\u6570\u636e\u8981\u6c42<\/b><\/strong>\uff1a<\/li><\/ul><\/li><\/ul><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li>\u521d\u59cb\u6a21\u578b\u5206\u8fa8\u7387\u9700\u8fbe\u5230\u4e2d\u7b49\u6c34\u5e73\uff08\u901a\u5e38\u4f18\u4e8e4\u00c5\uff09\u3002<\/li><li>\u6570\u636e\u91cf\u5145\u8db3\uff08&gt;50,000\u9897\u7c92\uff09\uff0c\u786e\u4fdd\u5c40\u90e8\u4f18\u5316\u7684\u7edf\u8ba1\u53ef\u9760\u6027\u3002<\/li><\/ul><\/li><li><b><\/b><strong><b>\u8ba1\u7b97\u8d44\u6e90<\/b><\/strong>\uff1a\u975e\u5747\u5300\u7ec6\u5316\u8ba1\u7b97\u91cf\u8f83\u5927\uff0c\u5efa\u8bae\u4f7f\u7528GPU\u52a0\u901f\uff08\u5982NVIDIA A100\/V100\uff09\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u53c2\u6570\u8bbe\u7f6e<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u5c40\u90e8\u533a\u57df\u5927\u5c0f<\/b><\/strong>\uff1a\u901a\u8fc7&#8211;patch-size\u8c03\u6574\u5b50\u4f53\u79ef\u5927\u5c0f\uff08\u9ed8\u8ba4\u503c\u53ef\u80fd\u4e3a10-20\u4f53\u7d20\uff09\uff0c\u9700\u6839\u636e\u76ee\u6807\u7ed3\u6784\u52a8\u6001\u6027\u8c03\u6574\u3002<\/li><li><b><\/b><strong><b>\u5e73\u6ed1\u7ea6\u675f\u5f3a\u5ea6<\/b><\/strong>\uff1a\u589e\u52a0\u5e73\u6ed1\u53c2\u6570\uff08\u5982&#8211;regularize\uff09\u53ef\u9632\u6b62\u5c40\u90e8\u8fc7\u62df\u5408\uff0c\u4f46\u53ef\u80fd\u964d\u4f4e\u5206\u8fa8\u7387\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) \u6d41\u7a0b\u793a\u4f8b<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u5747\u5300\u7ec6\u5316<\/b><\/strong>\uff1a\u5148\u901a\u8fc7Uniform Refinement\u83b7\u5f97\u4e2d\u7b49\u5206\u8fa8\u7387\u6a21\u578b\u3002<\/li><li><b><\/b><strong><b>\u8bca\u65ad\u5206\u6790<\/b><\/strong>\uff1a\u4f7f\u7528\u5c40\u90e8\u5206\u8fa8\u7387\u5de5\u5177\uff08\u5982CryoSPARC\u7684Local Resolution Estimation\uff09\u8bc6\u522b\u4f4e\u5206\u8fa8\u7387\u533a\u57df\u3002<\/li><li><b><\/b><strong><b>\u542f\u52a8Non-uniform Refinement<\/b><\/strong>\uff1a\u9488\u5bf9\u52a8\u6001\u533a\u57df\u4f18\u5316\uff0c\u76d1\u63a7\u5206\u8fa8\u7387\u63d0\u5347\u3002<\/li><li><b><\/b><strong><b>\u8fed\u4ee3\u4f18\u5316<\/b><\/strong>\uff1a\u6839\u636e\u7ed3\u679c\u8c03\u6574\u5c40\u90e8\u53c2\u6570\u6216\u91cd\u65b0\u5b9a\u4e49\u5173\u6ce8\u533a\u57df\u3002<\/li><\/ul><\/li><\/ul><p>\u00a0 \u00a0 \u00a0 5. <strong><b>\u5b9e\u9645\u6848\u4f8b<\/b><\/strong><\/p><p style=\"padding-left: 40px;\"><strong><b>(1) \u819c\u86cb\u767d\u7684\u8de8\u819c\u533a\u4f18\u5316<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u95ee\u9898<\/b><\/strong>\uff1a\u8de8\u819c\u533a\u7684\u758f\u6c34\u73af\u5883\u5bfc\u81f4\u5c40\u90e8\u8fd0\u52a8\u6027\u8f83\u9ad8\uff0c\u5747\u5300\u7ec6\u5316\u540e\u5206\u8fa8\u7387\u4e0d\u8db3\u3002<\/li><li><b><\/b><strong><b>\u89e3\u51b3\u65b9\u6848<\/b><\/strong>\uff1a\u975e\u5747\u5300\u7ec6\u5316\u72ec\u7acb\u6821\u6b63\u8de8\u819c\u533a\u7684B\u56e0\u5b50\uff0c\u4f7f\u03b1\u87ba\u65cb\u7684\u4fa7\u94fe\u6e05\u6670\u53ef\u89c1\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u75c5\u6bd2\u8863\u58f3\u9876\u70b9\u52a8\u6001\u6027<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u95ee\u9898<\/b><\/strong>\uff1a\u8863\u58f3\u9876\u70b9\u5b58\u5728\u5f00\u5408\u8fd0\u52a8\uff0c\u5168\u5c40\u7ec6\u5316\u6a21\u7cca\u4e86\u7ed3\u5408\u4f4d\u70b9\u7ec6\u8282\u3002<\/li><li><b><\/b><strong><b>\u7ed3\u679c<\/b><\/strong>\uff1a\u975e\u5747\u5300\u7ec6\u5316\u540e\uff0c\u9876\u70b9\u533a\u57df\u7684\u914d\u4f53\u7ed3\u5408\u53e3\u888b\u5206\u8fa8\u7387\u4ece5\u00c5\u63d0\u5347\u81f32.8\u00c5\u3002<\/li><\/ul><\/li><\/ul><p>\u00a0 \u00a0 \u00a0 6.<strong><b> \u6ce8\u610f\u4e8b\u9879<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u8fc7\u62df\u5408\u98ce\u9669<\/b><\/strong>\uff1a\u5c40\u90e8\u53c2\u6570\u8fc7\u591a\u53ef\u80fd\u5bfc\u81f4\u566a\u58f0\u88ab\u8bef\u8ba4\u4e3a\u4fe1\u53f7\uff0c\u9700\u901a\u8fc7\u4ea4\u53c9\u9a8c\u8bc1\uff08\u5982gold-standard FSC\uff09\u76d1\u63a7\u3002<\/li><li><b><\/b><strong><b>\u6570\u636e\u91cf\u4e0d\u8db3\u7684\u5c40\u9650<\/b><\/strong>\uff1a\u5c0f\u6570\u636e\u96c6\uff08&lt;30,000\u9897\u7c92\uff09\u53ef\u80fd\u65e0\u6cd5\u652f\u6301\u53ef\u9760\u7684\u5c40\u90e8\u4f18\u5316\u3002<\/li><li><b><\/b><strong><b>\u521d\u59cb\u6a21\u578b\u8d28\u91cf<\/b><\/strong>\uff1a\u4f4e\u8d28\u91cf\u521d\u59cb\u6a21\u578b\uff08\u5982\u5bf9\u79f0\u6027\u9519\u8bef\uff09\u4f1a\u5bfc\u81f4\u975e\u5747\u5300\u7ec6\u5316\u5931\u8d25\u3002<\/li><\/ul><\/li><\/ul><p><strong><b>\u603b\u7ed3<\/b><\/strong><\/p><p><strong><b>CryoSPARC\u7684Non-uniform Refinement\u9002\u7528\u4e8e\u4ee5\u4e0b\u573a\u666f<\/b><\/strong>\uff1a<\/p><ul><li>\u7ed3\u6784\u4e2d\u5b58\u5728<strong><b>\u5c40\u90e8\u52a8\u6001\u6027\u6216\u5f02\u8d28\u6027<\/b><\/strong>\uff08\u5982\u67d4\u6027\u533a\u57df\u3001\u914d\u4f53\u7ed3\u5408\u4f4d\u70b9\uff09\u3002<\/li><li>\u5747\u5300\u7ec6\u5316\u540e<strong><b>\u5206\u8fa8\u7387\u63d0\u5347\u505c\u6ede<\/b><\/strong>\uff0c\u9700\u7a81\u7834\u74f6\u9888\u3002<\/li><li>\u6837\u54c1\u5236\u5907\u5bfc\u81f4<strong><b>\u51b0\u5c42\u6216\u80cc\u666f\u4fe1\u53f7\u4e0d\u5747<\/b><\/strong>\uff0c\u9700\u5c40\u90e8\u6821\u6b63\u3002<\/li><\/ul><p>\u5176\u6838\u5fc3\u4ef7\u503c\u5728\u4e8e\u901a\u8fc7<strong><b>\u5c40\u90e8\u81ea\u9002\u5e94\u4f18\u5316<\/b><\/strong>\uff0c\u663e\u8457\u63d0\u5347\u590d\u6742\u751f\u7269\u5927\u5206\u5b50\u7ed3\u6784\u7684\u7ec6\u8282\u89e3\u6790\u80fd\u529b\uff0c\u662f\u51b7\u51bb\u7535\u955c\u9ad8\u5206\u8fa8\u7387\u91cd\u6784\u7684\u91cd\u8981\u5de5\u5177\u3002<\/p><p><span style=\"font-size: 14pt; color: #3366ff;\"><strong><b>\u56db\u3001<\/b><\/strong><strong><b>CryoSPARC\u4e2d\u7684heterogeneous refinement\u7684\u5e94\u7528\u573a\u666f<\/b><\/strong><\/span><\/p><p>CryoSPARC\u4e2d\u7684**Heterogeneous Refinement\uff08\u5f02\u8d28\u6027\u7ec6\u5316\uff09<strong><b>\u662f\u4e00\u79cd\u9488\u5bf9\u51b7\u51bb\u7535\u955c\u6570\u636e\u4e2d<\/b><\/strong>\u7ed3\u6784\u5f02\u8d28\u6027\uff08structural heterogeneity\uff09**\u7684\u9ad8\u7ea7\u4f18\u5316\u65b9\u6cd5\uff0c\u65e8\u5728\u901a\u8fc7\u5206\u79bb\u4e0d\u540c\u6784\u8c61\u72b6\u6001\u7684\u9897\u7c92\u5e76\u5206\u522b\u4f18\u5316\uff0c\u63d0\u5347\u5404\u4e9a\u7fa4\u7684\u7ed3\u6784\u5206\u8fa8\u7387\u3002\u4ee5\u4e0b\u662f\u5176\u6838\u5fc3\u5e94\u7528\u573a\u666f\u3001\u6280\u672f\u539f\u7406\u53ca\u4f7f\u7528\u7b56\u7565\u7684\u8be6\u7ec6\u5206\u6790\uff1a<\/p><ol><li><strong><b> \u9002\u7528\u573a\u666f<\/b><\/strong><\/li><\/ol><p style=\"padding-left: 40px;\"><strong><b>(1) \u591a\u6784\u8c61\u72b6\u6001\u5206\u79bb<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u52a8\u6001\u590d\u5408\u7269<\/b><\/strong>\uff1a\u4f8b\u5982\u9176\u5728\u50ac\u5316\u5faa\u73af\u4e2d\u7684\u4e0d\u540c\u72b6\u6001\uff08\u5f00\u653e\u6001\u3001\u95ed\u5408\u6001\uff09\u3001\u79bb\u5b50\u901a\u9053\u7684\u95e8\u63a7\u72b6\u6001\u3001\u5206\u5b50\u4f34\u4fa3\u4e0e\u5e95\u7269\u7ed3\u5408\u7684\u4e0d\u540c\u6784\u8c61\u7b49\u3002<\/li><li><b><\/b><strong><b>\u6df7\u5408\u6837\u54c1<\/b><\/strong>\uff1a\u82e5\u6837\u54c1\u4e2d\u5b58\u5728\u591a\u79cd\u6784\u8c61\uff08\u5982\u90e8\u5206\u9897\u7c92\u7ed3\u5408\u914d\u4f53\uff0c\u53e6\u4e00\u90e8\u5206\u672a\u7ed3\u5408\uff09\uff0c\u9700\u901a\u8fc7\u5f02\u8d28\u6027\u7ec6\u5316\u5206\u79bb\u5e76\u72ec\u7acb\u4f18\u5316\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u590d\u5408\u7269\u7684\u7ec4\u5206\u5dee\u5f02<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u4e9a\u57fa\u7f3a\u5931\u6216\u66ff\u6362<\/b><\/strong>\uff1a\u5982\u6838\u7cd6\u4f53\u4e0e\u4e0d\u540c\u7ffb\u8bd1\u56e0\u5b50\u7ed3\u5408\uff0c\u6216\u75c5\u6bd2\u8863\u58f3\u4e2d\u90e8\u5206\u4e9a\u57fa\u7f3a\u5931\u3002<\/li><li><b><\/b><strong><b>\u5171\u7eaf\u5316\u7684\u6c61\u67d3\u7269<\/b><\/strong>\uff1a\u5f53\u6837\u54c1\u4e2d\u6df7\u5165\u5176\u4ed6\u86cb\u767d\uff08\u5982\u4f34\u4fa3\u86cb\u767d\u6216\u964d\u89e3\u4ea7\u7269\uff09\uff0c\u9700\u901a\u8fc7\u7ec6\u5316\u5254\u9664\u65e0\u5173\u9897\u7c92\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) \u8fde\u7eed\u6784\u8c61\u53d8\u5316<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u67d4\u6027\u7ed3\u6784<\/b><\/strong>\uff1a\u5982\u819c\u86cb\u767d\u7684\u8de8\u819c\u533a\u6446\u52a8\u3001RNA\u805a\u5408\u9176\u7684\u6784\u8c61\u8fde\u7eed\u53d8\u5316\uff0c\u9700\u5c06\u8fde\u7eed\u5f02\u8d28\u6027\u5efa\u6a21\u4e3a\u79bb\u6563\u7684\u4e9a\u7fa4\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(4) \u914d\u4f53\u7ed3\u5408\u4f4d\u70b9\u7684\u5c40\u90e8\u5f02\u8d28\u6027<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u90e8\u5206\u7ed3\u5408<\/b><\/strong>\uff1a\u5f53\u914d\u4f53\u7ed3\u5408\u7387\u4e0d\u8db3100%\u65f6\uff08\u5982\u90e8\u5206\u9897\u7c92\u7ed3\u5408\u5c0f\u5206\u5b50\u836f\u7269\uff09\uff0c\u5f02\u8d28\u6027\u7ec6\u5316\u53ef\u5206\u79bb\u7ed3\u5408\/\u672a\u7ed3\u5408\u72b6\u6001\u3002<\/li><\/ul><\/li><\/ul><ol start=\"2\"><li><strong><b> \u6280\u672f\u539f\u7406<\/b><\/strong><\/li><\/ol><p style=\"padding-left: 40px;\"><strong><b>(1) \u591a\u6a21\u578b\u5e76\u884c\u4f18\u5316<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u540c\u6b65\u7ec6\u5316\u591a\u4e2a3D\u6a21\u578b<\/b><\/strong>\uff1a\u6bcf\u4e2a\u6a21\u578b\u5bf9\u5e94\u4e00\u79cd\u6784\u8c61\u72b6\u6001\uff0c\u7b97\u6cd5\u901a\u8fc7\u8fed\u4ee3\u66f4\u65b0\u6a21\u578b\u5e76\u91cd\u65b0\u5206\u7c7b\u9897\u7c92\u3002<\/li><li><b><\/b><strong><b>\u6982\u7387\u5206\u914d<\/b><\/strong>\uff1a\u6bcf\u4e2a\u9897\u7c92\u88ab\u5206\u914d\u5230\u6700\u5339\u914d\u7684\u6a21\u578b\uff08\u901a\u8fc7\u4f3c\u7136\u503c\u6700\u5927\u5316\uff09\uff0c\u652f\u6301\u8f6f\u5206\u7c7b\uff08\u5141\u8bb8\u9897\u7c92\u5c5e\u4e8e\u591a\u4e2a\u6a21\u578b\u7684\u6982\u7387\uff09\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u5206\u8fa8\u7387\u5bfc\u5411\u7684\u6743\u91cd\u8c03\u6574<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li>\u9ad8\u9891\u4fe1\u53f7\uff08\u9ad8\u5206\u8fa8\u7387\u7ec6\u8282\uff09\u4ec5\u5728\u4fe1\u566a\u6bd4\u8db3\u591f\u7684\u533a\u57df\u88ab\u4fdd\u7559\uff0c\u907f\u514d\u566a\u58f0\u6c61\u67d3\u4e0d\u540c\u6a21\u578b\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) \u52a8\u6001\u5206\u7c7b\u7b56\u7565<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li>\u521d\u59cb\u9636\u6bb5\u4f7f\u7528\u8f83\u5c11\u7684\u7c7b\u522b\uff08\u59822-4\u7c7b\uff09\u6355\u6349\u4e3b\u8981\u5dee\u5f02\uff0c\u540e\u7eed\u9010\u6b65\u589e\u52a0\u7c7b\u522b\u4ee5\u7ec6\u5206\u5fae\u5c0f\u6784\u8c61\u53d8\u5316\u3002<\/li><\/ul><\/li><\/ul><ol start=\"3\"><li><strong><b> \u4e0eClass3D\u7684\u5bf9\u6bd4<\/b><\/strong><\/li><\/ol><table style=\"width: 48.865%;\"><tbody><tr><td style=\"width: 19.6635%;\"><strong><b>\u7279\u5f81<\/b><\/strong><\/td><td style=\"width: 37.0146%;\"><strong><b>Heterogeneous Refinement<\/b><\/strong><\/td><td style=\"width: 97.9452%;\"><strong><b>Class3D<\/b><\/strong><\/td><\/tr><tr><td style=\"width: 19.6635%;\"><strong><b>\u76ee\u6807<\/b><\/strong><\/td><td style=\"width: 37.0146%;\">\u9ad8\u5206\u8fa8\u7387\u7ec6\u5316\u5df2\u5206\u7c7b\u7684\u4e9a\u7fa4<\/td><td style=\"width: 97.9452%;\">\u521d\u6b65\u5206\u7c7b\uff08\u4f4e\u5206\u8fa8\u7387\u6784\u8c61\u5206\u79bb\uff09<\/td><\/tr><tr><td style=\"width: 19.6635%;\"><strong><b>\u8f93\u5165\u8981\u6c42<\/b><\/strong><\/td><td style=\"width: 37.0146%;\">\u9700\u521d\u59cb\u5206\u7c7b\u7ed3\u679c\u6216\u9ad8\u8d28\u91cf\u521d\u59cb\u6a21\u578b<\/td><td style=\"width: 97.9452%;\">\u53ef\u76f4\u63a5\u4ece\u539f\u59cb\u9897\u7c92\u5f00\u59cb<\/td><\/tr><tr><td style=\"width: 19.6635%;\"><strong><b>\u8ba1\u7b97\u91cf<\/b><\/strong><\/td><td style=\"width: 37.0146%;\">\u66f4\u9ad8\uff08\u591a\u6a21\u578b\u5e76\u884c\u4f18\u5316\uff09<\/td><td style=\"width: 97.9452%;\">\u8f83\u4f4e<\/td><\/tr><tr><td style=\"width: 19.6635%;\"><strong><b>\u5206\u8fa8\u7387\u6f5c\u529b<\/b><\/strong><\/td><td style=\"width: 37.0146%;\">\u53ef\u8fbe\u8fd1\u539f\u5b50\u7ea7\uff08&lt;3\u00c5\uff09<\/td><td style=\"width: 97.9452%;\">\u901a\u5e38\u9650\u5236\u5728\u4e2d\u7b49\u5206\u8fa8\u7387\uff08~4-6\u00c5\uff09<\/td><\/tr><\/tbody><\/table><ol start=\"4\"><li><strong><b> \u4f7f\u7528\u5efa\u8bae<\/b><\/strong><\/li><\/ol><p style=\"padding-left: 40px;\"><strong><b>(1) \u8f93\u5165\u6570\u636e\u51c6\u5907<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u521d\u59cb\u5206\u7c7b<\/b><\/strong>\uff1a\u5efa\u8bae\u5148\u7528Class3D\u62162D\u5206\u7c7b\u521d\u6b65\u5206\u79bb\u5f02\u8d28\u6027\u9897\u7c92\uff0c\u518d\u8f93\u5165\u5230Heterogeneous Refinement\u3002<\/li><li><b><\/b><strong><b>\u6a21\u578b\u521d\u59cb\u5316<\/b><\/strong>\uff1a\u53ef\u4e3a\u6bcf\u4e2a\u7c7b\u522b\u63d0\u4f9b\u72ec\u7acb\u7684\u521d\u59cb\u6a21\u578b\uff08\u5982\u901a\u8fc7Class3D\u751f\u6210\u7684\u591a\u4e2a\u4f4e\u5206\u8fa8\u7387\u6a21\u578b\uff09\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u53c2\u6570\u8bbe\u7f6e<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u7c7b\u522b\u6570\uff08&#8211;K\uff09<\/b><\/strong>\uff1a\u6839\u636e\u5f02\u8d28\u6027\u590d\u6742\u5ea6\u9009\u62e9\uff08\u901a\u5e382-4\u7c7b\uff09\uff0c\u8fc7\u591a\u7c7b\u522b\u53ef\u80fd\u5bfc\u81f4\u8fc7\u62df\u5408\u3002<\/li><li><b><\/b><strong><b>\u5c40\u90e8\u63a9\u6a21\uff08&#8211;mask\uff09<\/b><\/strong>\uff1a\u82e5\u5f02\u8d28\u6027\u96c6\u4e2d\u5728\u5c40\u90e8\u533a\u57df\uff08\u5982\u7ed3\u5408\u53e3\u888b\uff09\uff0c\u4f7f\u7528\u5c40\u90e8\u63a9\u6a21\u63d0\u5347\u5206\u7c7b\u7075\u654f\u5ea6\u3002<\/li><li><b><\/b><strong><b>\u5bf9\u79f0\u6027\u7ea6\u675f\uff08&#8211;sym\uff09<\/b><\/strong>\uff1a\u5bf9\u5bf9\u79f0\u6027\u7ed3\u6784\uff08\u5982\u75c5\u6bd2\u8863\u58f3\uff09\u4fdd\u6301\u5bf9\u79f0\u7ea6\u675f\uff0c\u907f\u514d\u6a21\u578b\u504f\u79bb\u771f\u5b9e\u6784\u8c61\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) \u6d41\u7a0b\u793a\u4f8b<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u521d\u6b65\u5206\u7c7b<\/b><\/strong>\uff1a\u4f7f\u7528Class3D\u5206\u79bb\u51fa2-3\u4e2a\u4e3b\u8981\u6784\u8c61\u7c7b\u522b\u3002<\/li><li><b><\/b><strong><b>\u542f\u52a8Heterogeneous Refinement<\/b><\/strong>\uff1a\u8f93\u5165\u5206\u7c7b\u540e\u7684\u9897\u7c92\u548c\u5bf9\u5e94\u521d\u59cb\u6a21\u578b\u3002<\/li><li><b><\/b><strong><b>\u76d1\u63a7\u6536\u655b<\/b><\/strong>\uff1a\u901a\u8fc7\u5206\u8fa8\u7387\uff08FSC\uff09\u3001\u9897\u7c92\u5206\u5e03\u6bd4\u4f8b\u548c\u5bc6\u5ea6\u56fe\u5dee\u5f02\u5224\u65ad\u662f\u5426\u7a33\u5b9a\u3002<\/li><li><strong><b>\u8fed\u4ee3\u8c03\u6574<\/b><\/strong>\uff1a\u82e5\u67d0\u4e9b\u7c7b\u522b\u672a\u6536\u655b\uff0c\u53ef\u5408\u5e76\u5197\u4f59\u7c7b\u522b\u6216\u589e\u52a0\u8ba1\u7b97\u8fed\u4ee3\u6b21\u6570\u3002<\/li><\/ul><\/li><\/ul><p>\u00a0 \u00a0 \u00a05. <strong><b>\u5b9e\u9645\u6848\u4f8b<\/b><\/strong><\/p><p style=\"padding-left: 40px;\"><strong><b>(1) \u6838\u7cd6\u4f53\u7ffb\u8bd1\u590d\u5408\u7269<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u95ee\u9898<\/b><\/strong>\uff1a\u6838\u7cd6\u4f53\u4e0e\u5ef6\u4f38\u56e0\u5b50\uff08EF-G\/Tu\uff09\u7ed3\u5408\u65f6\u5b58\u5728\u591a\u79cd\u6784\u8c61\uff0c\u5168\u5c40\u7ec6\u5316\u5bfc\u81f4\u5bc6\u5ea6\u6a21\u7cca\u3002<\/li><li><b><\/b><strong><b>\u89e3\u51b3\u65b9\u6848<\/b><\/strong>\uff1aHeterogeneous Refinement\u5206\u79bb\u51faEF-G\u7ed3\u5408\uff08\u65cb\u8f6c\u72b6\u6001\uff09\u548c\u672a\u7ed3\u5408\u7c7b\u522b\uff0c\u5206\u522b\u7ec6\u5316\u81f33.2\u00c5\u548c3.5\u00c5\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u79bb\u5b50\u901a\u9053\u7684\u95e8\u63a7\u72b6\u6001<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u95ee\u9898<\/b><\/strong>\uff1a\u819c\u86cb\u767d\u5728\u5f00\u653e\/\u95ed\u5408\u6001\u4e4b\u95f4\u52a8\u6001\u5e73\u8861\uff0c\u5355\u6a21\u578b\u7ec6\u5316\u65e0\u6cd5\u533a\u5206\u3002<\/li><li><b><\/b><strong><b>\u7ed3\u679c<\/b><\/strong>\uff1a\u5206\u79bb\u51fa\u5f00\u653e\u6001\uff08\u5b54\u5f84\u6269\u5927\uff09\u548c\u95ed\u5408\u6001\uff08\u5b54\u5f84\u6536\u7f29\uff09\uff0c\u5206\u8fa8\u7387\u63d0\u5347\u81f38\u00c5\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) \u90e8\u5206\u914d\u4f53\u7ed3\u5408\u7684\u9176<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u95ee\u9898<\/b><\/strong>\uff1a\u4ec530%\u9897\u7c92\u7ed3\u5408\u6291\u5236\u5242\uff0c\u5355\u6a21\u578b\u7ec6\u5316\u5e73\u5747\u5316\u540e\u914d\u4f53\u5bc6\u5ea6\u7f3a\u5931\u3002<\/li><li><b><\/b><strong><b>\u89e3\u51b3\u65b9\u6848<\/b><\/strong>\uff1a\u5f02\u8d28\u6027\u7ec6\u5316\u5206\u79bb\u7ed3\u5408\/\u672a\u7ed3\u5408\u7c7b\u522b\uff0c\u7ed3\u5408\u7c7b\u4e2d\u6e05\u6670\u663e\u793a\u914d\u4f53\u5bc6\u5ea6\uff082.5\u00c5\uff09\u3002<\/li><\/ul><\/li><\/ul><ol start=\"6\"><li><strong><b> \u6ce8\u610f\u4e8b\u9879<\/b><\/strong><br \/><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u6570\u636e\u91cf\u9700\u6c42<\/b><\/strong>\uff1a\u6bcf\u4e2a\u7c7b\u522b\u81f3\u5c11\u9700\u898110,000-20,000\u9897\u7c92\u4ee5\u4fdd\u8bc1\u7edf\u8ba1\u663e\u8457\u6027\u3002<\/li><li><b><\/b><strong><b>\u8fc7\u62df\u5408\u98ce\u9669<\/b><\/strong>\uff1a\u7c7b\u522b\u8fc7\u591a\u6216\u6a21\u578b\u5dee\u5f02\u8fc7\u5c0f\u53ef\u80fd\u5bfc\u81f4\u865a\u5047\u5206\u7c7b\uff0c\u9700\u901a\u8fc7\u4ea4\u53c9\u9a8c\u8bc1\uff08\u5982gold-standard FSC\uff09\u9a8c\u8bc1\u3002<\/li><li><b><\/b><strong><b>\u8ba1\u7b97\u8d44\u6e90<\/b><\/strong>\uff1a\u591a\u6a21\u578b\u5e76\u884c\u4f18\u5316\u9700\u5927\u91cfGPU\u8d44\u6e90\uff08\u5efa\u8bae\u4f7f\u7528\u591a\u5361\u5e76\u884c\uff09\u3002<\/li><\/ul><\/li><\/ol><p><strong><b>\u603b\u7ed3<\/b><\/strong><\/p><p><strong><b>CryoSPARC\u7684Heterogeneous Refinement\u9002\u7528\u4e8e\u4ee5\u4e0b\u573a\u666f<\/b><\/strong>\uff1a<\/p><ul><li>\u6837\u54c1\u4e2d\u5b58\u5728<strong><b>\u79bb\u6563\u7684\u591a\u6784\u8c61\u72b6\u6001<\/b><\/strong>\uff08\u5982\u7ed3\u5408\/\u672a\u7ed3\u5408\u3001\u5f00\u653e\/\u95ed\u5408\uff09\u3002<\/li><li>\u9700\u8981<strong><b>\u9ad8\u5206\u8fa8\u7387\u5206\u79bb\u52a8\u6001\u590d\u5408\u7269<\/b><\/strong>\u7684\u4e9a\u7fa4\u7ed3\u6784\u3002<\/li><li>\u521d\u6b65\u5206\u7c7b\u663e\u793a\u660e\u663e\u5f02\u8d28\u6027\u4f46\u5206\u8fa8\u7387\u4e0d\u8db3\u3002<\/li><\/ul><p>\u5176\u6838\u5fc3\u4ef7\u503c\u5728\u4e8e\u901a\u8fc7<strong><b>\u591a\u6a21\u578b\u534f\u540c\u4f18\u5316<\/b><\/strong>\uff0c\u5c06\u5f02\u8d28\u6027\u4ece\u201c\u5e72\u6270\u56e0\u7d20\u201d\u8f6c\u5316\u4e3a\u201c\u89e3\u6790\u76ee\u6807\u201d\uff0c\u663e\u8457\u63d0\u5347\u590d\u6742\u52a8\u6001\u7ed3\u6784\u7684\u89e3\u6790\u80fd\u529b\uff0c\u662f\u51b7\u51bb\u7535\u955c\u9ad8\u5206\u8fa8\u7387\u7814\u7a76\u4e2d\u4e0d\u53ef\u6216\u7f3a\u7684\u5de5\u5177\u3002<\/p><p><span style=\"font-size: 14pt; color: #3366ff;\"><strong><b>\u4e94\u3001<\/b><\/strong><strong><b>\u5982\u4f55\u7406\u89e3\u5206\u8fa8\u7387\uff08resolution\uff09\u4ee5\u53caFSC<\/b><\/strong><\/span><\/p><p>\u5728\u51b7\u51bb\u7535\u955c\uff08cryo-EM\uff09\u548c\u7ed3\u6784\u751f\u7269\u5b66\u4e2d\uff0c**\u5206\u8fa8\u7387\uff08resolution\uff09<strong><b>\u548c<\/b><\/strong>\u5085\u91cc\u53f6\u58f3\u5c42\u76f8\u5173\u7cfb\u6570\uff08Fourier Shell Correlation, FSC\uff09**\u662f\u8bc4\u4f30\u4e09\u7ef4\u91cd\u6784\u8d28\u91cf\u7684\u6838\u5fc3\u6307\u6807\u3002\u5b83\u4eec\u7684\u5b9a\u4e49\u3001\u8ba1\u7b97\u65b9\u5f0f\u53ca\u76f8\u4e92\u5173\u7cfb\u5982\u4e0b\uff1a<\/p><ol><li><strong><b> \u5206\u8fa8\u7387\uff08Resolution\uff09<\/b><\/strong><\/li><\/ol><p style=\"padding-left: 40px;\"><strong><b>(1) \u5b9a\u4e49<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u5206\u8fa8\u7387<\/b><\/strong>\u8868\u793a\u4e09\u7ef4\u91cd\u6784\u6a21\u578b\u80fd\u591f\u6e05\u6670\u533a\u5206\u76f8\u90bb\u7ed3\u6784\u7ec6\u8282\u7684\u6700\u5c0f\u8ddd\u79bb\uff0c\u901a\u5e38\u4ee5**\u00c5ngstr\u00f6m\uff08\u00c5\uff09**\u4e3a\u5355\u4f4d\u3002\u4f8b\u5982\uff1a<\/li><\/ul><\/li><\/ul><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li><b><\/b><strong><b>3\u00c5\u5206\u8fa8\u7387<\/b><\/strong>\uff1a\u53ef\u5206\u8fa8\u6c28\u57fa\u9178\u4fa7\u94fe\uff08\u5982\u82ef\u73af\u7684\u516d\u5143\u73af\uff09\u3002<\/li><li><b><\/b><strong><b>4-5\u00c5\u5206\u8fa8\u7387<\/b><\/strong>\uff1a\u53ef\u89c1\u03b1\u87ba\u65cb\u548c\u03b2\u6298\u53e0\u7684\u8d70\u5411\uff0c\u4f46\u4fa7\u94fe\u7ec6\u8282\u6a21\u7cca\u3002<\/li><li><b><\/b><strong><b>&gt;8\u00c5\u5206\u8fa8\u7387<\/b><\/strong>\uff1a\u4ec5\u80fd\u8bc6\u522b\u86cb\u767d\u8d28\u6574\u4f53\u7684\u5f62\u72b6\u6216\u5927\u5c3a\u5ea6\u7279\u5f81\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u7269\u7406\u610f\u4e49<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u5206\u8fa8\u7387\u8d8a\u9ad8<\/b><\/strong>\uff0c\u7ed3\u6784\u7ec6\u8282\u8d8a\u6e05\u6670\uff0c\u53cd\u6620\u5b9e\u9a8c\u4e2d\u4fe1\u53f7\u7684\u9ad8\u9891\u6210\u5206\uff08\u5bf9\u5e94\u5c0f\u5c3a\u5ea6\u7279\u5f81\uff09\u88ab\u6709\u6548\u6355\u6349\u3002<\/li><li><b><\/b><strong><b>\u5206\u8fa8\u7387\u53d7\u9650\u4e8e<\/b><\/strong>\uff1a<\/li><\/ul><\/li><\/ul><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li><b><\/b><strong><b>\u6570\u636e\u8d28\u91cf<\/b><\/strong>\uff1a\u4fe1\u566a\u6bd4\uff08SNR\uff09\u3001\u9897\u7c92\u6570\u91cf\u3001\u51b0\u5c42\u5747\u5300\u6027\u3002<\/li><li><b><\/b><strong><b>\u8fd0\u52a8\u6a21\u7cca<\/b><\/strong>\uff1a\u6837\u54c1\u5728\u7535\u5b50\u675f\u4e0b\u7684\u6f02\u79fb\u6216\u7ed3\u6784\u672c\u8eab\u7684\u67d4\u6027\u3002<\/li><li><b><\/b><strong><b>\u7b97\u6cd5\u9650\u5236<\/b><\/strong>\uff1a\u91cd\u6784\u8fc7\u7a0b\u4e2d\u53c2\u6570\u4f18\u5316\u662f\u5426\u5145\u5206\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) \u5206\u8fa8\u7387\u5206\u7c7b<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u5168\u5c40\u5206\u8fa8\u7387<\/b><\/strong>\uff1a\u6574\u4e2a\u7ed3\u6784\u7684\u5e73\u5747\u5206\u8fa8\u7387\uff08\u7531FSC\u8ba1\u7b97\uff09\u3002<\/li><li><b><\/b><strong><b>\u5c40\u90e8\u5206\u8fa8\u7387<\/b><\/strong>\uff1a\u7ed3\u6784\u4e0d\u540c\u533a\u57df\u7684\u5206\u8fa8\u7387\u5dee\u5f02\uff08\u5982\u67d4\u6027\u533a\u57df\u5206\u8fa8\u7387\u8f83\u4f4e\uff09\u3002<\/li><\/ul><\/li><\/ul><ol start=\"2\"><li><strong><b> \u5085\u91cc\u53f6\u58f3\u5c42\u76f8\u5173\u7cfb\u6570\uff08FSC\uff09<\/b><\/strong><\/li><\/ol><p style=\"padding-left: 40px;\"><strong><b>(1) \u5b9a\u4e49<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>FSC<\/b><\/strong>\u662f\u4e00\u79cd\u901a\u8fc7\u5085\u91cc\u53f6\u7a7a\u95f4\u5206\u6790\u6765\u91cf\u5316\u4e09\u7ef4\u91cd\u6784\u6a21\u578b\u4e00\u81f4\u6027\u7684\u7edf\u8ba1\u65b9\u6cd5\u3002\u5176\u6838\u5fc3\u601d\u60f3\u662f\uff1a<\/li><\/ul><\/li><\/ul><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li>\u5c06\u6570\u636e\u96c6\u968f\u673a\u5206\u4e3a<strong><b>\u4e24\u4e2a\u72ec\u7acb\u5b50\u96c6<\/b><\/strong>\uff08\u5982\u534a\u6570\u636e\u96c61\u548c\u534a\u6570\u636e\u96c62\uff09\u3002<\/li><li>\u5206\u522b\u7528\u8fd9\u4e24\u4e2a\u5b50\u96c6\u91cd\u6784<strong><b>\u4e24\u4e2a\u534a\u6a21\u578b\uff08half-map 1\u548chalf-map 2\uff09<\/b><\/strong>\u3002<\/li><li>\u8ba1\u7b97\u4e24\u4e2a\u534a\u6a21\u578b\u5728\u5085\u91cc\u53f6\u7a7a\u95f4\u4e2d\u4e0d\u540c\u7a7a\u95f4\u9891\u7387\uff08\u5373\u4e0d\u540c\u5206\u8fa8\u7387\u58f3\u5c42\uff09\u4e0a\u7684<strong><b>\u76f8\u5173\u7cfb\u6570<\/b><\/strong>\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) FSC\u8ba1\u7b97<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u516c\u5f0f<\/b><\/strong>\uff1a<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\">FSC(r)=\u2211k\u2208\u58f3\u5c42rF1(k)\u22c5F2\u2217(k)\u2211k\u2208\u58f3\u5c42r\u2223F1(k)\u22232\u22c5\u2211k\u2208\u58f3\u5c42r\u2223F2(k)\u22232FSC(<em><i>r<\/i><\/em>)=\u2211<strong><b>k<\/b><\/strong>\u2208\u58f3\u5c42<em><i>r<\/i><\/em>\u200b\u2223<em><i>F<\/i><\/em>1\u200b(<strong><b>k<\/b><\/strong>)\u22232\u22c5\u2211<strong><b>k<\/b><\/strong>\u2208\u58f3\u5c42<em><i>r<\/i><\/em>\u200b\u2223<em><i>F<\/i><\/em>2\u200b(<strong><b>k<\/b><\/strong>)\u22232\u200b\u2211<strong><b>k<\/b><\/strong>\u2208\u58f3\u5c42<em><i>r<\/i><\/em>\u200b<em><i>F<\/i><\/em>1\u200b(<strong><b>k<\/b><\/strong>)\u22c5<em><i>F<\/i><\/em>2\u2217\u200b(<strong><b>k<\/b><\/strong>)\u200b<\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li>F1<em><i>F<\/i><\/em>1\u200b\u548cF2<em><i>F<\/i><\/em>2\u200b\uff1a\u4e24\u4e2a\u534a\u6a21\u578b\u7684\u5085\u91cc\u53f6\u53d8\u6362\u3002<\/li><li>r<em><i>r<\/i><\/em>\uff1a\u7a7a\u95f4\u9891\u7387\u5bf9\u5e94\u7684\u5206\u8fa8\u7387\u58f3\u5c42\u3002<\/li><\/ul><\/li><li><b><\/b><strong><b>\u66f2\u7ebf\u793a\u4f8b<\/b><\/strong>\uff1a<br \/><ul style=\"list-style-type: circle;\"><li>\u9ad8\u9891\u533a\u57df\uff08\u9ad8\u5206\u8fa8\u7387\uff09FSC\u503c\u4e0b\u964d\uff0c\u53cd\u6620\u566a\u58f0\u5360\u6bd4\u589e\u52a0\u3002<\/li><li>\u5206\u8fa8\u7387\u9608\u503c\u901a\u5e38\u53d6<strong><b>FSC=0.143<\/b><\/strong>\uff08\u57fa\u4e8e\u566a\u58f0\u7406\u8bba\u7684\u6807\u51c6\uff09\u6216<strong><b>FSC=0.5<\/b><\/strong>\uff08\u66f4\u4fdd\u5b88\u7684\u9608\u503c\uff09\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) FSC\u7684\u4f5c\u7528<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>\u8bc4\u4f30\u5206\u8fa8\u7387<\/b><\/strong>\uff1a\u786e\u5b9a\u5168\u5c40\u5206\u8fa8\u7387\uff08\u5982FSC=0.143\u5bf9\u5e94\u7684\u9891\u7387\uff09\u3002<\/li><li><b><\/b><strong><b>\u9a8c\u8bc1\u6a21\u578b\u53ef\u9760\u6027<\/b><\/strong>\uff1a\u82e5FSC\u66f2\u7ebf\u9aa4\u964d\uff0c\u8bf4\u660e\u9ad8\u9891\u4fe1\u53f7\u53ef\u4fe1\uff1b\u82e5\u66f2\u7ebf\u5e73\u7f13\uff0c\u53ef\u80fd\u8fc7\u62df\u5408\u3002<\/li><li><b><\/b><strong><b>\u8bca\u65ad\u95ee\u9898<\/b><\/strong>\uff1a<\/li><\/ul><\/li><\/ul><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li><b><\/b><strong><b>FSC\u66f2\u7ebf\u8fc7\u65e9\u4e0b\u964d<\/b><\/strong>\uff1a\u6570\u636e\u8d28\u91cf\u5dee\u6216\u8fd0\u52a8\u6a21\u7cca\u4e25\u91cd\u3002<\/li><li><b><\/b><strong><b>FSC\u66f2\u7ebf\u5728\u4f4e\u9891\u9707\u8361<\/b><\/strong>\uff1a\u5bf9\u79f0\u6027\u9519\u8bef\u6216\u521d\u59cb\u6a21\u578b\u504f\u5dee\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><ol start=\"3\"><li><strong><b> \u5206\u8fa8\u7387\u4e0eFSC\u7684\u5173\u7cfb<\/b><\/strong><br \/><ul style=\"list-style-type: disc;\"><li><b><\/b><strong><b>FSC\u51b3\u5b9a\u5206\u8fa8\u7387<\/b><\/strong>\uff1a\u901a\u8fc7FSC\u66f2\u7ebf\u4e0e\u9608\u503c\u7684\u4ea4\u70b9\u5b9a\u4e49\u5206\u8fa8\u7387\uff08\u4f8b\u5982\uff0cFSC=0.143\u5bf9\u5e943.2\u00c5\uff09\u3002<\/li><li><b><\/b><strong><b>\u5206\u8fa8\u7387\u4f9d\u8d56FSC\u7684\u7edf\u8ba1\u663e\u8457\u6027<\/b><\/strong>\uff1aFSC\u503c\u8d8a\u9ad8\u7684\u533a\u57df\uff0c\u4fe1\u53f7\u8d8a\u53ef\u9760\u3002<\/li><li><b><\/b><strong><b>\u793a\u4f8b<\/b><\/strong>\uff1a<\/li><\/ul><\/li><\/ol><ul><li style=\"list-style-type: none;\"><ul><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li>\u82e5FSC\u57281\/3.0\u00c5\u207b\u00b9\u5904\u964d\u81f3143\uff0c\u5219\u5206\u8fa8\u7387\u4e3a3.0\u00c5\u3002<\/li><li>\u82e5FSC\u57281\/4.0\u00c5\u207b\u00b9\u5904\u5df2\u4f4e\u4e8e143\uff0c\u5219\u5206\u8fa8\u7387\u4e0d\u8d85\u8fc74.0\u00c5\u3002<\/li><\/ul><\/li><\/ul><\/li><\/ul><ol start=\"4\"><li><strong><b> \u5b9e\u9645\u5e94\u7528\u4e2d\u7684\u6ce8\u610f\u4e8b\u9879<\/b><\/strong><\/li><\/ol><p style=\"padding-left: 40px;\"><strong><b>(1) \u5206\u8fa8\u7387\u9608\u503c\u7684\u9009\u62e9<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul><li><b><\/b><strong><b>FSC=0.143<\/b><\/strong>\uff1a\u5e7f\u6cdb\u4f7f\u7528\u7684\u6807\u51c6\uff0c\u57fa\u4e8e\u201c\u566a\u58f0\u76f8\u5173\u7406\u8bba\u201d\uff0c\u9002\u7528\u4e8e\u9ad8\u4fe1\u566a\u6bd4\u6570\u636e\u3002<\/li><li><b><\/b><strong><b>FSC=0.5<\/b><\/strong>\uff1a\u66f4\u4fdd\u5b88\u7684\u6807\u51c6\uff0c\u5e38\u7528\u4e8e\u5ba1\u7a3f\u8981\u6c42\u6216\u4f4e\u4fe1\u566a\u6bd4\u6570\u636e\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(2) \u8fc7\u62df\u5408\u7684\u68c0\u6d4b<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul><li><b><\/b><strong><b>\u91d1\u6807\u51c6FSC\uff08Gold-standard FSC\uff09<\/b><\/strong>\uff1a\u901a\u8fc7\u72ec\u7acb\u534a\u6570\u636e\u96c6\u8ba1\u7b97\uff0c\u907f\u514d\u8fc7\u62df\u5408\u3002<\/li><li><b><\/b><strong><b>\u6a21\u578b\u4e0e\u6570\u636e\u7684FSC<\/b><\/strong>\uff1a\u82e5\u7528\u5168\u90e8\u6570\u636e\u91cd\u6784\u7684\u6a21\u578b\u4e0e\u534a\u6570\u636e\u8ba1\u7b97\u7684FSC\uff0c\u53ef\u80fd\u9ad8\u4f30\u5206\u8fa8\u7387\u3002<\/li><\/ul><\/li><\/ul><p style=\"padding-left: 40px;\"><strong><b>(3) \u5c40\u90e8\u5206\u8fa8\u7387\u5206\u6790<\/b><\/strong><\/p><ul><li style=\"list-style-type: none;\"><ul><li>\u4f7f\u7528\u5c40\u90e8FSC\u6216\u5757\u72b6FSC\uff08\u5982<strong><b>ResMap<\/b><\/strong>\u3001<strong><b>CryoSPARC Local Resolution<\/b><\/strong>\uff09\u8bc4\u4f30\u7ed3\u6784\u4e0d\u540c\u533a\u57df\u7684\u5206\u8fa8\u7387\u5dee\u5f02\u3002<\/li><\/ul><\/li><\/ul><ol start=\"5\"><li><strong><b> \u793a\u4f8b\u56fe\u89e3<\/b><\/strong><b><\/b><\/li><\/ol><ul style=\"list-style-type: disc;\"><li><strong><b>\u9ad8\u5206\u8fa8\u7387\u7ed3\u6784\uff088\u00c5\uff09<\/b><\/strong>\uff1a<br \/><ul style=\"list-style-type: circle;\"><li>FSC\u66f2\u7ebf\u7f13\u6162\u4e0b\u964d\uff0c\u57281\/2.8\u00c5\u207b\u00b9\u5904\u4ea4\u4e8e143\u3002<\/li><li>\u5bc6\u5ea6\u56fe\u4e2d\u53ef\u89c1\u6e05\u6670\u7684\u4fa7\u94fe\uff08\u5982\u916a\u6c28\u9178\u7684\u82ef\u73af\uff09\u3002<\/li><\/ul><\/li><li><b><\/b><strong><b>\u4e2d\u7b49\u5206\u8fa8\u7387\u7ed3\u6784\uff085\u00c5\uff09<\/b><\/strong>\uff1a<br \/><ul style=\"list-style-type: circle;\"><li>FSC\u66f2\u7ebf\u57281\/4.5\u00c5\u207b\u00b9\u5904\u4ea4\u4e8e143\u3002<\/li><li>\u4ec5\u53ef\u89c1\u03b1\u87ba\u65cb\u8d70\u5411\uff0c\u4fa7\u94fe\u65e0\u6cd5\u5206\u8fa8\u3002<\/li><\/ul><\/li><li><b><\/b><strong><b>\u4f4e\u5206\u8fa8\u7387\u7ed3\u6784\uff088\u00c5\uff09<\/b><\/strong>\uff1a<\/li><li style=\"list-style-type: none;\"><ul style=\"list-style-type: circle;\"><li>FSC\u66f2\u7ebf\u5728\u4f4e\u9891\u5373\u9aa4\u964d\uff0c\u4ea4\u4e8e0.143\u65f6\u5bf9\u5e948\u00c5\u3002<\/li><li>\u4ec5\u80fd\u8bc6\u522b\u86cb\u767d\u8d28\u6574\u4f53\u5f62\u72b6\u3002<\/li><\/ul><\/li><\/ul><p><strong><b>\u603b\u7ed3<\/b><\/strong><\/p><ul><li><b><\/b><strong><b>\u5206\u8fa8\u7387<\/b><\/strong>\u662f\u7ed3\u6784\u7ec6\u8282\u7684\u5ea6\u91cf\uff0c\u76f4\u63a5\u51b3\u5b9a\u6a21\u578b\u7684\u751f\u7269\u5b66\u89e3\u91ca\u80fd\u529b\u3002<\/li><li><b><\/b><strong><b>FSC<\/b><\/strong>\u662f\u8bc4\u4f30\u5206\u8fa8\u7387\u7684\u5ba2\u89c2\u6807\u51c6\uff0c\u901a\u8fc7\u5206\u6790\u4e24\u4e2a\u72ec\u7acb\u534a\u6a21\u578b\u7684\u4e00\u81f4\u6027\uff0c\u907f\u514d\u4e3b\u89c2\u504f\u5dee\u3002<\/li><li>\u4e8c\u8005\u7684\u5173\u7cfb\u662f\u51b7\u51bb\u7535\u955c\u6570\u636e\u5904\u7406\u7684\u57fa\u77f3\uff1a<strong><b>\u6ca1\u6709\u53ef\u9760\u7684FSC\uff0c\u5206\u8fa8\u7387\u58f0\u660e\u5c06\u7f3a\u4e4f\u79d1\u5b66\u4e25\u8c28\u6027<\/b><\/strong>\u3002\u5728\u5b9e\u9645\u7814\u7a76\u4e2d\uff0c\u9700\u7ed3\u5408FSC\u66f2\u7ebf\u3001\u5bc6\u5ea6\u56fe\u8d28\u91cf\u53ca\u751f\u5316\u9a8c\u8bc1\uff0c\u5168\u9762\u8bc4\u4f30\u7ed3\u6784\u6a21\u578b\u7684\u53ef\u9760\u6027\u3002<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>\u52a0\u5165\u884c\u4e1a\u9886\u8896\uff0c\u53c2\u52a0\u4e9a\u6d32\u6700\u5927\u7684\u4ece\u9972\u6599\u5230\u98df\u54c1\u7684\u6d3b\u52a8\u3002\u8be5\u6d3b\u52a8\u4e13\u6ce8\u4e8e\u755c\u7267\u4e1a\u751f\u4ea7\u3001\u52a8\u7269\u86cb\u767d\u751f\u4ea7\u4ee5\u53ca\u6240\u6709\u76f8\u5173\u9886\u57df\uff0c\u5982\u9972\u6599\u751f\u4ea7\u3001\u52a8\u7269\u517b\u6b96\u3001\u8089\u7c7b\u3001\u9c7c\u7c7b\u3001\u867e\u7c7b\u3001\u86cb\u7c7b\u3001\u4e73\u5236\u54c1\u7b49\u7684\u52a0\u5de5\u3002\u53d1\u73b0\u65b0\u673a\u4f1a\uff0c\u4e0e\u5173\u952e\u53c2\u4e0e\u8005\u5efa\u7acb\u8054\u7cfb\uff0c\u5728\u4e0d\u65ad\u53d1\u5c55\u53d8\u5316\u7684\u5168\u7403\u98df\u54c1\u8d38\u6613\u683c\u5c40\u4e2d\u4fdd\u6301\u9886\u5148\u5730\u4f4d\u3002<\/p>","protected":false},"author":1,"featured_media":351,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[7],"tags":[],"class_list":["post-115","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-local"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/posts\/115","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/comments?post=115"}],"version-history":[{"count":6,"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/posts\/115\/revisions"}],"predecessor-version":[{"id":872,"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/posts\/115\/revisions\/872"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/media\/351"}],"wp:attachment":[{"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/media?parent=115"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/categories?post=115"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.drtem.cn\/en\/wp-json\/wp\/v2\/tags?post=115"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}